When a stray black cat named Kuro crosses Mahiru Shirota's path, the high school freshman's life will never be the same again. Kuro is, in fact, no ordinary feline, but a servamp: a servant vampire. While Mahiru's personal philosophy is one of non-intervention, he soon becomes embroiled in an ancient, altogether surreal conflict between vampires and humans.

The Servamps and their pact-bound "Eves" are finally getting back to their normal lives as they recover from their injuries from the previous battles. However, when it starts snowing in the middle of summer, one of the Eves, Mahiru Shirota, suspects vampiric interference. Concerned by the strange phenomenon, he sets out to gather the group once more to try and solve the mystery; however, they suddenly lose contact with Misono Arisuin, the Eve of the Servamp of Lust. This movie delves into the untold past of Misono and his brother Mikuni Arisuin, as well as the many mysteries of the grand Arisuin Mansion.